Investigation into the Use of Short-Column Reversed-Phase LC in the Determination of log P Values

Aim To investigate the possibility of determining log P via Reversed-Phase LC Introduction Increasingly, the stopping point of low concentrations of active ingredients in complex mixtures, sold for tender consumption, has become more necessary. Regulations book imposed strict limits on the type and concentrations of a military of substances sold as foods or drugs. Such requirements demand uninflected techniques that be fast and unquestionable and combine the breakup and analysis steps in a single operation. Chromatography is the most wide used technique for the analysis of non-inorganic mixtures. Gas chromatography (where the savour must(prenominal) be volatilized) and liquid chromatography (where the specimen can be fixed in the liquid state) are the most common methods in general use. High action Liquid Chromatography (HPLC) is the method of excerption whenever the sample cannot easily be converted to the gas stage Theory The figure above depicts the main compone nts of a young HPLC system. In HPLC, a solution containing the compounds of interest is injected into a loop which has been calibrated to contain a specified volume. The valve faulting is then rotated, allowing a sample stream of mobile phase (eluent) to sweep the sample from the loop onto a column, packed with a suitable unmoving phase, where the separation occurs.

The eluent is delivered from a pump at a immutable rate, at a mechanical press sufficiently lofty to bounce back the backpressure of the column. The separation efficiency is inversely relative to the particle surface of the column packing material. High pressures are required to ride a liquid through a tightly-packed column change with small particle material,! and the availability of high school pressure solvent delivery systems is directly responsible for the high carrying into action. Assuming that a suitable column has been elect for the separation of interest, all components should pass through the column and wash... If you regard to get a full essay, order it on our website: OrderEssay.net

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